Biochemistry. Lab Book for Students

ABOUT THE BOOK

Biochemistry lies at the heart of life’s most fundamental questions: What defines life? How do cells communicate, grow, and adapt? It is the study of the molecular machinery that underpins biological processes, bridging the gap between biology and chemistry. From understanding the structures of biomolecules to unraveling the intricate pathways of metabolism and genetics, biochemistry illuminates the dynamic and complex systems that sustain life.

This practice book is designed for students pursuing studies in health sciences, offering essential guidance for mastering laboratory techniques and methods, bridging the gap between theory and application.

It aims to provide the tools to reinforce the understanding and sharpening the skills in biochemistry for future specialists for fields such as Biochemistry, Chemistry, Cell Biology, Genetics, Microbiology, and more. The manual is structured into two main parts: the first section introduces basic methods used in the initial training of students, such as volumetric analysis and identification techniques. These foundational skills are critical for building confidence and accuracy in laboratory work. The second section focuses on methods for determining various blood parameters, alongside discussions of their interpretation and clinical significance. This section bridges laboratory practice with real-world applications, emphasizing the relevance of biochemistry in health sciences.

By combining theoretical principles with hands-on practice, this manual provides a comprehensive resource for developing technical expertise and critical thinking. Each experiment and technique is presented with clear instructions, ensuring accessibility for students at all levels. Additionally, the manual highlights the importance of accuracy, precision, and safety in the laboratory environment.

Through this guide, students will gain the foundational and advanced skills necessary for a career in health sciences, fostering a deeper understanding of the biochemical processes and analytical techniques essential to their field.

Authors

TABLE OF CONTENT

LAB SAFETY RULES/7

SOLUTIONS/11

pH OF THE SOLUTIONS/13

BUFFER SYSTEMS/19

VOLUMETRIC ANALYSIS/24

AMINO ACIDS REACTIONS/30

CHEMICAL PROPERTIES OF THE PROTEINS/35

DETERMINATION OF UREASE ACTIVITY/40

EFFECT OF DIFFERENT FACTORS ON ENZYMES ACTIVITY/42

NUCLEIC ACIDS/46

MINERALS/50

REACTIONS OF CARBOHYDRATES/59

DETERMINATION OF PLASMA GLUCOSE CONCENTRATION/66

DETERMINATION OF TOTAL PROTEIN CONCENTRATION/69

PLASMA ENZYMES/73

THE LIPID COMPOUNDS/82

BILIRUBIN/87

UREA/91

CREATININE/94

URINALYSIS/96

REFERENCES/102